Structural and Dynamics Aspects of ASC Speck Assembly

Dynamics of in vivo ASC speck formation

Activated danger or pathogen sensors trigger assembly of the inflammasome adaptor ASC into specks, large signaling platforms considered hallmarks of inflammasome activation. Because a lack of in vivo tools has prevented the study of endogenous ASC dynamics, we generated a live ASC reporter through CRISPR/Cas9 tagging of the endogenous gene in zebrafish. We see strong ASC expression in the skin ...

Correction: Dynamics of in vivo ASC speck formation

Zebrafish earns its stripes for in vivo ASC speck dynamics

Assembly of the ASC speck is critical for signaling by the inflammasome. In this issue, Kuri et al. (2017. J. Cell Biol. https://doi.org/10.1083/jcb.201703103) use live microscopy to track fluorescently tagged endogenous ASC in the zebrafish, describing the molecular domains driving ASC speck assembly and identifying a key role for macrophages in ASC speck removal in vivo.

Dynamics of ASC speck formation during skin inflammatory responses in vivo

17 Activated danger or pathogen sensors trigger assembly of the inflammasome adaptor ASC into 18 specks, large signalling platforms considered hallmarks of inflammasome activation. Because a 19 lack of in vivo tools has prevented the study of endogenous ASC dynamics, we generated a live 20 ASC reporter through CRISPR/Cas9 tagging of the endogenous gene in zebrafish. We see strong 21 ASC express...

Activation of the NLRP1b inflammasome independently of ASC-mediated caspase-1 autoproteolysis and speck formation

Despite its clinical importance in infection and autoimmunity, the activation mechanisms of the NLRP1b inflammasome remain enigmatic. Here we show that deletion of the inflammasome adaptor ASC in BALB/c mice and in C57BL/6 macrophages expressing a functional NLRP1b prevents anthrax lethal toxin (LeTx)-induced caspase-1 autoproteolysis and speck formation. However, ASC(-/-) macrophages undergo n...